Sepax SRT-C SEC Columns,Sepax Technologies, Inc._specification/price/image

Product Detail
Company Profile

SRT®-C SEC phase, developed based on innovative surface coating technology, comprises of uniform, hydrophilic, and neutral nanometer thick film chemically bonded to high purity and mechanically stabilized silica. SRT®-C, uses lay-down monolayer on porous silica as oppose to SRT of stand-up monolayer (as shown below), offers ideal phase chemistry for hydrophobic samples like insulin, membrane protein monoclonal antibodies derivatized with polymer branches (polypeptide or PEG) separation.

Stationary Phase Structure Illustration and Comparison

Phase structure difference: a monolayer stands up on the silica surface for SRT® and Zenix™, and a monolayer lays down on the silica surface for SRT®-C and Zenix™-C.

Sepax SEC Product Family Comparison Chart

Product Line	SRT®	Zenix™	SRT®-C	Zenix™-C
Particle size	5 µm	3 µm	5 µm	3 µm
Pore size (Å)	100, 150, 300, 500, 1000 & 2000	100, 150, 300	100, 150, 300, 500, 1000 & 2000	100, 150, 300
Resolution	High	Highest, Short column for faster separation	High	Highest, Short column for faster separation
Efficiency	High	Doubled from 5µm	High	Doubled from 5µm
Selectivity	Same for SRT® and Zenix™		Same for SRT®-C and Zenix™-C
Surface structure	Chemically bonded stand-up monolayer		Chemically bonded lay-down monolayer
Recommended Sample Types	Monoclonal antibodies, proteins, peptides, nucleic acids, oligonucleotides, virus, and water-soluble polymers		"Tough samples" such as hydrophobic proteins like insulin, membrane protein monoclonal antibodies derivatized with polymer branches, e.g. polypeptide, PEG.

Packing

SRT®-C Technical Specifications

Phase	SRT®-C SEC-150	SRT®-C SEC-300	SRT®-C SEC-500
Material	Neutral, hydrophilic film bonded silica
Particle size	5 µm
Pore size	~ 150 Å	~ 300 Å	~ 500 Å
Protein MW range (native)	500 - 150,000	5,000 - 1,250,000	15,000 - 5,000,000
pH stability	2 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
Backpressure (for a 7.8×300 mm)	~ 700 psi	~ 700 psi	~ 700 psi
Maximum backpressure	~ 4,500 psi	~ 3,500 psi	~ 3,000 psi
Salt concentration range	20 mM - 2.0 M
Maximum temperature	~ 80 °C
Mobile phase compatibility	Aqueous and organic

Characteristic

Ideal for separation and analysis of hydrophobic proteins, and monoclonal antibodies derivatized with polymer branches such as polypeptide or PEG
Particle size of 5 µm
Selection of pore size: 100, 150, 300, 500, 1,000, and 2,000 Å
High separation efficiency and resolution
High capacity
High lot-to-lot reproducibility
High protein recovery with intact biological activity
Negligible non-specific interactions

Separation of a protein mixture by SRT-C SEC-300 column.

Conditions
Columns:	SRT-C SEC-300(5 µm, 7.8×300mm)
Mobile phase:	150 mM Sodium Phosphate, pH 7.0
Flow rate:	1.0 mL/min
Temperature:	Ambient (~23 °C)
Detection:	UV 214 nm
Injection volume:	10 µL
Samples:	1) Thyroglobulin, 670kD 2) γ-Globulin, 158kD 3) Ovalbumin, 44kD 4) Myoglobin, 17.6kD 5) Poly-DL-alanine (1-5 kD) 6) Uracil, 120D

Protein MW calibration with elution volume for SRT-C phases.

Conditions
Columns:	SRT-C (5 µm, 7.8×300mm)
Mobile phase:	150 mM Sodium Phosphate, pH 7.0
Flow rate:	1.0 mL/min
Detection:	UV 214 nm
Injection volume:	10 µL
Samples:	1. Thyroglobulin, 670kD 2. γ-Globulin, 158kD 3. BSA, 66kD 4. Ovalbumin, 44kD 5. Myoglobin, 17.6kD 6. Ribonuclease A, 13.7kD 7. B12, 1.35kD 8. Uracil, 120D