Sepax Zenix-C SEC Columns,Sepax Technologies, Inc._specification/price/image

Product Detail
Company Profile

Description

Zenix™-C SEC phase, developed based on innovative surface coating technology, comprises of uniform, hydrophilic, and neutral nanometer thick film chemically bonded to high purity and mechanically stabilized silica. Zenix™-C, uses lay-down monolayer on porous silica as oppose to Zenix of stand-up monolayer (as shown below), offers ideal phase chemistry for hydrophobic samples like insulin, membrane protein monoclonal antibodies derivatized with polymer branches (polypeptide or PEG) separation. The 3µm based packing material allows for high resolution and performance separation.

Packing

Stationary Phase Structure Illustration and Comparison

Phase structure difference: a monolayer stands up on the silica surface for SRT® and Zenix™, and a monolayer lays down on the silica surface for SRT®-C and Zenix™-C.

Sepax SEC Product Family Comparison Chart

Product Line	SRT®	Zenix™	SRT®-C	Zenix™-C
Particle size	5 µm	3 µm	5 µm	3 µm
Pore size (Å)	100, 150, 300, 500, 1000 & 2000	80, 100, 150, 300	100, 150, 300, 500, 1000 & 2000	80, 100, 150, 300
Resolution	High	Highest, Short column for faster separation	High	Highest, Short column for faster separation
Efficiency	High	Doubled from 5µm	High	Doubled from 5µm
Selectivity	Same for SRT® and Zenix™		Same for SRT®-C and Zenix™-C
Surface structure	Chemically bonded stand-up monolayer		Chemically bonded lay-down monolayer
Recommended Sample Types	Monoclonal antibodies, proteins, peptides, nucleic acids, oligonucleotides, virus, and water-soluble polymers		"Tough samples" such as hydrophobic proteins like insulin, membrane protein monoclonal antibodies derivatized with polymer branches, e.g. polypeptide, PEG.

Zenix-C Technical Specifications

Phase	Zenix-C SEC-100	Zenix-C SEC-150	Zenix-C SEC-300
Material	Neutral, hydrophilic film bonded silica
Particle size	3 µm
Pore size	~ 100 Å	~ 150 Å	~ 300 Å
Protein MW range (native)	100 - 100,000	500 - 150,000	5,000 - 1,250,000
pH stability	2 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
Backpressure (for a 7.8×300 mm)	~ 1,500 psi	~ 1,375 psi	~ 1,100 psi
Backpressure (for a 4.6×300 mm)	~ 1,400 psi	~ 1,250 psi	~ 1,000 psi
Maximum backpressure	~ 4,500 psi	~ 4,500 psi	~ 3,500 psi
Salt concentration range	20 mM - 2.0 M
Maximum temperature	~ 80 °C
Mobile phase compatibility	Aqueous and organic

Characteristic

Ideal for separation and analysis of hydrophobic proteins, and monoclonal antibodies derivatized with polymer branches such as polypeptide or PEG
Particle size of 3 µm
Selection of pore size: 100, 150 and 300 Å
Highest separation efficiency and resolution
High capacity
High lot-to-lot reproducibility
High protein recovery with intact biological activity
Negligible non-specific interactions

Separation of a protein mixture by Zenix-C SEC-300 column.

Conditions
Columns:	Zenix-C SEC-300(3 µm, 7.8×300mm)
Mobile phase:	150 mM Sodium Phosphate, pH 7.0
Flow rate:	1.0 mL/min
Temperature:	Ambient (~23 °C)
Detection:	UV 214 nm
Injection volume:	10 µL
Samples:	1) Thyroglobulin, 670kD 2) γ-Globulin, 158 kD 3) Ovalbumin, 44kD 4) Myoglobin, 17.6 kD 5) Poly-DL-alanine (1-5 kD) 6) B12, 1.35 kD

Separation of Biorad protein mixture by Zenix-C SEC 100, 150 and 300 columns.

Conditions
Columns:	Zenix-C (3 µm, 7.8×300mm)
Mobile phase:	150 mM Sodium Phosphate, pH 7.0
Flow rate:	1.0 mL/min
Detection:	UV 214 nm
Injection volume:	10 µL
Samples:	1) Thyroglobulin, 670kD 2) γ-Globulin, 158 kD 3) Ovalbumin, 44kD 4) Myoglobin, 17.6 kD 5) B12, 1.35 kD

Separation of protein mixture A by Zenix-C SEC 100, 150 and 300 columns.

Conditions
Columns:	Zenix-C (3 µm, 7.8×300mm)
Mobile phase:	150 mM Sodium Phosphate, pH 7.0
Temperature:	Ambient (~23 °C)
Flow rate:	1.0 mL/min
Detection:	UV 214 nm
Injection volume:	10 µL
Samples:	1) Thyroglobulin, 670kD 2) BSA, 66kD 3) Ribonuclease A, 13.7kD 4) Uracil , 120D

Protein molecular weight vs elution volume is plotted in below figure, indicating Zenix-C 100, 150 and 300 have large linear elution region.

Protein MW calibration with elution volume for Zenix-C phases.

Conditions
Columns:	3 µm, 7.8×300mm
Mobile phase:	150 mM Sodium Phosphate, pH 7.0
Flow rate:	1.0 mL/min
Detection:	UV 214 nm
Injection volume:	10 µL
Samples:	1. Thyroglobulin, 670 kD 2. γ-Globulin, 158 kD 3. BSA, 66 kD 4. Ovalbumin, 44 kD 5. Myoglobin, 17.6 kD 6. Ribonuclease A, 13.7 kD 7. B12, 1.35 kD 8. Uracil, 120