Product Specification
For researchers and laboratories focused on human immunology and cytokine analysis, the Human IL-4 One-Step ELISA Kit from Antbio Inc. stands out as a reliable, high-performance tool designed to streamline the quantitative detection of human Interleukin-4 (IL-4) in biological samples.
IL-4 is a key Th2 cytokine that plays pivotal roles in regulating humoral immunity, allergic responses, and the differentiation of immune cells. Accurate measurement of IL-4 levels is critical for studies in immunology, allergy research, autoimmune diseases, and drug development—making this kit an essential asset for advancing scientific discoveries.
This one-step ELISA kit is engineered for efficiency without compromising precision. It integrates optimized reagents and a simplified protocol, eliminating complex intermediate steps to reduce assay time while ensuring exceptional sensitivity and specificity. The kit is validated for use with common sample types including serum, plasma, and cell culture supernatants, offering flexibility for diverse experimental needs. Each component undergoes strict quality control to guarantee batch-to-batch consistency, minimizing variability and ensuring reliable, reproducible results.
Backed by Antbio Inc.’s expertise in developing life science reagents, this kit combines technical excellence with user-friendly design, suitable for both experienced researchers and laboratory technicians.
| Antigen |
IL-4 |
| Immunogen |
Recombinant Protein |
| Antibody Type |
Recombinant mAb |
| Reactivity |
Hu |
| Purification |
Protein A |
| Stability & Storage |
2 to 8℃ as supplied.
|
Kit
| Precision |
Intra-assay: 4.1%；
Inter-assay: 4.4% |
| Sample type |
Cell culture supernatant, Serum, Plasma |
| Assay type |
Sandwich (quantitative) |
| Sensitivity |
0.435 pg/ml |
| Range |
1.25 pg/mL -80 pg/mL |
| Recovery |
Cell culture supernatant: 87%
Serum: 111%
Plasma: 116% |
| Assay time |
60 minutes |
| Species reactivity |
Human |
| NIBSC convert |
The dose response curve of the International Standard (88/656) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (88/656) units, use the following equation: NIBSC (88/656) approximate value (IU/mL) = 0.01 x OneStep Human IL-4 value (pg/mL). |
Background
Interleukin 4(IL4) was first identified as a helper T cell product with the capacity to co-stimulate B cell growth in vitro. IL-4 also can rescue B-cells from apoptosis, enhancing their survival, and is responsible for immunoglobulin isotype switching to IgG1 and IgE. The effect of IL-4 signaling is mediated through the IL-4 receptor alpha chain (IL-4R伪). Upon binding to its ligand, IL-4R伪 dimerizes either with the common gamma chain (纬c) to produce the type-1 signaling complex located mainly on hematopoietic cells, or with the IL-13 receptor alpha 1 (IL-13Rα) to produce the type-2 complex, which is expressed also on non-hematopoietic cells. The type-1 signaling complex is critical for Th2-skewing of T cells and the development of alternatively activated macrophages (AAMΦs), while the type-2 complex plays a role in non-hematopoietic responses to IL-4 and IL-13.
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