Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human IL-6 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-6 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-6 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-6 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.02pg/ml

Detection Range: 0.31-20 pg/ml

Recovery Range: 74-119%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 6 (IL-6)It is a cytokine with both pro-inflammatory and anti-inflammatory properties. In humans, it is encoded by the IL-6 gene. During infection and after trauma, especially inflammation caused by burn or other tissue damage, IL-6 will be secreted by T cells and macrophages to promote the immune response. Studies have shown that IL-6 is required for mice to resist Streptococcus pneumoniae infection, indicating that IL-6 plays a role in resisting infection.

IL-6 can promote the process of inflammatory and autoimmune diseases in many diseases, such as diabetes, atherosclerosis, depression, Alzheimer's disease, systemic lupus erythematosus, multiple myeloma, prostate cancer, Behcet's disease and rheumatoid arthritis. Patients with advanced / metastatic cancer have higher levels of IL-6 in their blood.