Mouse CCL3/MIP-1α ELISA Kit,Absin_specification/price/image

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Product Specification

Description

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. Anti mouse mip-1α The monoclonal antibody was coated on the microplate, and mip-1&alpha in samples and standards; It will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti mouse mip-1&alpha was added; Polyclonal antibody, and mip-1&alpha bound on the microplate; Combine to form immune complexes, and the free components are washed away; Add the substrate solution (chromogenic agent), the color of the solution gradually turns blue, add the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Precoated 96 well plates, standards, anti mouse mip-1α One copy of detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.5 pg/ml

Detection Range: 7.81 - 500 pg/ml

Recovery Range: 85.0-111.9%

Storage Method: 2-8 ℃

Standard Curve

Background:

MIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily, which was initially purified from the conditioned medium of LPS stimulated murine macrophage cell lines. Mip-1& alpha; is highly effective on a variety of cells (including monocytes, T cells, B cells, and eosinophils)Plays a chemotactic role.
two human mip-1α Genes are generated by duplication / mutation. They encode mip-1α Isoforms CCL3 / ld78a and CCL3L1 / ld78b, which share 94% amino acid sequence homology. Human CCL3 / ld78a is a single copy gene, while human CCL3L1 / ld78b gene copy number varies among populations. Human CCL3L1 / ld78b binds and signals through chemokine receptors CCR1, CCR5. Compared with CCL3 / ld78a, CCL3L1 / ld78b has higher binding affinity for CCR5, which is also a co receptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.