Human IL-17/IL-17A ELISA Kit,Absin_specification/price/image

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Product Specification

Description

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. Anti human IL-17 monoclonal antibody is coated on the microplate. IL-17 in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human IL-17 multi antibody was added to combine with IL-17 bound on the microplate to form an immune complex, and the free components were washed away; Add the substrate solution (chromogenic agent), the color of the solution gradually turns blue, add the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Pre coated 96 well plate, standard, anti human IL-17 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.8 pg/ml

Detection Range: 15.6 - 1000 pg/ml

Recovery Range: 82-110%

Storage Method: 2-8 ℃

Standard Curve

Background:

The IL-17 family is composed of at least six pro-inflammatory cytokines, which share a conserved cysteine knot structure, but differentiate at the N terminus. IL-17 family members are glycoproteins secreted as dimers, which can induce the production of local cytokines and recruit granulocytes to the site of inflammation. IL-17 is composed of IL-17 and il-23-induced, mainly in activated cd4+ T cells, unlike Th1 or Th2 cells. IL-17F has the strongest homology with IL-17, but is only induced by IL-23 in activated monocytes. IL-17B binds to the IL-17B receptor, but not to the IL-17 receptor, and has the strongest homology with il-17d, which is expressed by resting cd4+ T cells and cd19+ B cells. Il-17e is mainly produced by Th2 cells and recruits eosinophils to lung tissue. The expression pattern of il-17c is very limited, but it has been detected in adult prostate and fetal kidney banks.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.