Rat IL-4 ELISA Kit,Absin_specification/price/image

Product Detail
Company Profile

Product Specification

Synonym

B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Description

Testing Principle

This kit uses quantitative sandwich enzyme immunoassay technique. A specific anti-rat IL-4 antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the IL-4 present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification

Sensitivity: 2.3 pg/mL

Detection range: 15.6 - 1,000 pg/mL

Recovery rate: 75-112%

Storage: 2-8 ° C

The standard curve:

Background: IL-4

Il-4 is a secretory protein of 129 amino acids.Il-4 is a cytokine produced mainly by activated T lymphocytes, mast cells and basophils.By acting on a variety of cell types, IL-4 regulates a variety of immune responses. Il-4 is involved in several B cell activation processes and induces class II MHC molecules to be expressed in dormant B cells.It is also a common irritant in DNA synthesis. Il-4 can enhance the secretion and cell surface expression of IgE and IgG1.It also regulates the expression of low affinity Fc receptors for IgE (CD23) on lymphocytes and monocytes.Il-4 positively regulates the expression of IL31RA in macrophages.

Technical hints: For research use only, not for in vitro diagnosis.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.