| Usage | 1. Perform extraction as soon as possible after specimen collection, following the relevant literature. Experiments should be performed as soon as possible after extraction. If experiments cannot be performed immediately, the specimens can be stored at -20°C, but repeated freezing and thawing should be avoided.
2. Samples containing NaN3 cannot be tested, as NaN3 inhibits horseradish peroxidase (HRP) activity.
Procedure 1. Dilution of Standard: This kit provides one standard sample at full strength. Users can dilute the sample in a small test tube according to the following chart. 2000pg/ml | Standard No. 5 | Add 150μl of the original standard to 150μl of the standard diluent | 1000pg/ml | Standard No. 4 | Add 150μl of the original standard to 150μl of the standard diluent | Standard No. 3 | Add 150μl of Standard No. 4 to 150μl of Standard Diluent | 250pg/ml | Standard No. 2 | Add 150μl of Standard No. 3 to 150μl of Standard Diluent | | 250pg/ml | Standard No. 2 | Add 150μl of Standard No. 3 to 150μl of Standard Diluent | 125pg/ml | | Serial number | Component name | Specification | Serial number | Component name | Specification | 1 | 30 times concentrated detergent | 20ml×1 bottle | 6 | Developer B solution | 6ml×1/bottle | 2 | Enzyme Labeled Reagent | 6ml×1 bottle | 7 | Stop Solution | 6ml×1 bottle | 3 | Enzyme-coated plate | 12 wells×8 strips | 8 | Standard (4000pg/ml) | 0.5ml×1 bottle | 4 | Sample diluent | 6ml×1 bottle | 9 | Standard diluent | 1.5ml×1 bottle | 5 | Developer A Liquid | 6ml×1 bottle | 11 | Sealing film | 2 photos |
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| General Notes | 1. After removing the kit from the refrigerator, allow it to equilibrate at room temperature for 1 hour before use. If the enzyme-coated plate is opened and not fully used, store it in a sealed bag. 2. Crystals may form in the concentrated wash buffer. Warming in a water bath during dilution can aid dissolution. Washing does not affect the results. 3. Use a pipette for each sample addition step and frequently calibrate its accuracy to avoid experimental error. The time for each addition should ideally be within 5 minutes. For large numbers of samples, using a dispenser is recommended. 4. Develop a standard curve with each measurement, preferably in duplicate. If the analyte content in the sample is too high (the OD value of the sample is greater than the OD value of the first standard well), dilute the sample a certain number of times (n) with sample diluent before measurement. When calculating the total dilution factor (×n×5), multiply the final dilution factor. 5. The sealing film is for single use only to prevent cross-contamination. 6. Please store the substrate in the dark. 7. Strictly follow the instructions for operation. The test results must be determined based on the readings of the microplate reader. 8. All samples, washing solutions and various wastes should be treated as infectious materials. 9. Components of different batches of this reagent must not be mixed. |
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