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Dengue lgM ELISA
Dengue lgM ELISA
Place of Origin:
China
Brand:
SPAN
Model:
Price:
Hits:
25 
Updated:
4/25/2025
  • Product Detail
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     For in vitro diagnostic and professional use only.

    INTENDED USE 96TESTS/KIT
    The Dengue IgM ELISA is a solid-phase enzyme-linked immunosorbent assay for the qualitative detection of anti dengue virus (DEN1, 2, 3, 4) IgM in human serum or plasma. It is intended for healthcare professional use only as an aid in the diagnosis of acute infection with dengue virus. PRINCIPLE The Dengue IgM ELISA is a solid-phase enzyme-linked immunosorbent assay based on the principle of the capture immunoassaymethodology for thedetection ofIgM anti-dengue virus in human serum or plasma. During the assay, the test specimen is first incubated in the coated microwe l. Anti-dengue IgM, if present in the specimen, binds to the anti-human IgM antibodies coated on the microwel surface, and any unbound specimen is then removed by a wash step. During a second incubation with HRP-dengue Conjugate working solution, the anti dengue IgM absorbed on the surface of microwel binds to the conjugate through dengue antigen, forming a conjugate complex. Unbound conjugate is then removed by washing. After addition of the TMB substrate, the presence of the conjugate complex is shown by development of a blue color resulting from a reaction between the enzyme and substrate. This reaction is then quenched by addition of the Stop Solution, and the absorbance value for each microwe lisdetermined usinga spectrophotometer at 450/620-690 nm. SUMMARY Dengue virus is an enveloped, single-stranded, positive-sense RNA virus that belongs to the Flaviviridae family and can be classified into four distinct serotypes (DEN1, 2, 3, 4). The virus is transmitted by daytime-biting mosquitoes, principa ly Aedes aegypti and Aedes albopictus.Currently, more than 2.5 billion people living in tropical and subtropical areas of Asia, Africa, Australia, and the Americas are at risk for dengue infection. An estimated 67-136 million cases of dengue fever and 20,000 deaths occur annualy on a worldwide basis. Serological detection is a common method for the diagnosis of infection with dengue virus. During a primary infection, anti-dengue virus IgM starts to appear approximately 4-6 days after the onset of fever, peaks after approximately two weeks, and remains in circulation for about 2-3 months. Anti-dengue virus IgG levels increase slowly, peak around 14-21 days, and then decrease to low levels, persisting for life. During secondary infection, IgM antibodies increase simultaneously with, or fo lowing the IgG secondary antibodies, which is a strong and rapid response, and can be detected as early as three days after the onset of symptoms. IgM antibody levels occur, in general, at a lower titer than that of IgG. Importantly, during secondary infection, IgM antibody levels are significantly lower in comparison with primary infection. The Dengue IgM ELISA uses recombinant dengue virus antigens to specificaly detect IgM antibodies against a l four denguevirus serotypes (DEN1, 2, 3 and4).
    bio-equip.cn
    Span Biotech Ltd. is a research based company for rapid tests, with strong support from National Key Laboratory of Technology Projects of 10th and 11th Five-Year Plan and Faculty of Life Sciences of HuBei University. SpanBio also housed a R&D team that is developing gene recombination, cell cultivation and protein purification techniques. SpanBio pays strict attention on rapid tests for human being, animal diseases and food safety detection. It provides a number of customized services to professional distributors and partnering affiliates with excellent quality, competitive prices and super service.
    Efficiently structured to provide lasting and economic health care solutions with export ready goods, our main products cover the following immunodiagnostic rapid test kits:
    Rapid Tests for Human Use :
    1) Fertility: Pregnancy HCG, ovulation LH, menopause FSH
    2) Infectious diseases: Hepatitis B, HCV, HIV
    3) Drug abuse: AMP, BAR, BZO, COC, mAMP, MOP, MTD, OPI, PCP, TCA, THC
    4) Tumor markers: CEA, PSA, AFP, FOB
    5) STDs(Sexually Transmitted Diseases): Gonorrhea, syphilis, chlamydia
    6) New products: Malaria, H. Pylori, Multi-Hepatis B Panel (HBsAg, Anti-HBs, HBeAg, Anti-HBe, Anti-HBc), Blood Glucose Test, Multi-drug of Abuse Panel, Avian (Bird) Flu Rapid Test, etc.
    Rapid Tests for Veterinary :
    1) Bovine: Cow Pregnancy , Cow Ovulation, Bovine Brucella, Bovine Tuberculosis ,Food and Mouth Disease NSP Ab Test
    2) Canine: Canine Distemper, Canine Parvovirus, Canine Coronavirus
    3) Feline : Feline Leukemia Virus Ag Rapid Test ,Feline Panleucopenia Virus Ag Test,Feline Immunodeficiency Virus Ab Test
    Rapid Tests for Food Safety :
    1)For Antibiotics Residue : Nitrofurans (AOZ,AMOZ, AHD, SEM), Streptomycin , Sulfonamides , Chloramphenicol , Quinolone , Beta-Lactam&Tetracycline Combo (2 in 1), β-Lactams & Streptomycin & Chloramphenicol & Tetracyclines Combo (4 in 1), Beta-Lactam, Tetracycline test
    2) For Beta-Agonist Residue : Ractopamine, Clenbuterol ,Salbutamol
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