Introduction
This product contains F5 Taq DNA Polymerase, dNTP, and an optimized buffer system. It only needs to add primers and templates to perform amplification reaction, thereby reducing pipetting operations and improving detection throughput and reproducibility of results. The amplification system contains protective agents that keep 2 × Master Mix stable in activity after repeated freezing and thawing. This product containing loading buffer, so PCR products can be directly loaded for electrophoresis after the reaction, which is convenient to use. The PCR product has an adenine at the 3' end that can be
cloned into the T vector. It is also compatible with TOPO cloning kits.
Component
| MEL1004-1 |
2× F5 Taq PCR Master Mix |
1 mL |
| MEL1004-2 |
2× F5 Taq PCR Master Mix |
5× 1 mL |
| MEL1004-2 |
2× F5 Taq PCR Master Mix |
100× 1 mL |
Notes for Operation
Taq DNA Polymerase has a certain reactivity at room temperature, please prepare the PCR system on ice, and then place it on the PCR machine for the reaction. This can reduce non-specific amplification that occurs during the reaction preparation stage, and help to obtain highly specific amplification results.
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