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- 5X Concentrate COOMASSIE nano Protein Staining Solution
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5X Concentrate COOMASSIE nano ╴Innovatively developed Next-Generation Protein Stain for faster and environment-friendly gel visualization.
5X COOMASSIE nano, innovatively enhanced by the proprietary nano-technology, is a concentrated, easy-to-reconstitute protein staining solution for SDS-PAGE gels and is also compatible with mass spectrophotometry. Its next generation formula offers faster protein detection and higher sensitivity while eliminating the conventional need for destaining and wash. In the absence of hazardous substances, such as methanol and acetic acid, 5X COOMASSIE nano is considered to be safe and environmentally friendly by reducing carbon footprint by as much as 80%.
Figure 1. The same performance between 1X & 5X COOMASSIE nano. We used ImageJ to analysis the 85 kDa band of lane 2 (red arrow), comparing the band intensity between 1X and 5X Coomassie nano. The variation is under 10%.
Lane 1：Prestain Protein Ladder #PM019-0500
Lane 2-15：2X Dilutions of Unstain Ladder #PMU12-0500
Figure 2. COOMASSIEnano Protein Staining Solution practices the fast-signal representing at 5 min. Prestained protein ladder, unstain protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS-PAGE (4-20% Novex Gel), please remove the gel from the cassette then proceed to submerge the gel in proper amount of COOMASSIEnano dye, enough to cover the gel. Lightly agitate the staining box for 5 minutes to over night at room temperature.
Figure 3. COOMASSIEnano Protein Staining Solution demonstrates the high sensitivity detection could be down to 10 ng (Lane H). Prestained protein ladder, unstained protein ladder, and serial diluted BSA were prepared and applied in electrophoresis. After running 10% homemade gel (0.75 mm thickness), please remove the gel from the cassette then proceed to submerge the gel in a proper amount of COOMASSIEnano Protein Staining Solution, enough to cover the gel. Lightly agitate the staining container at room temperature when staining for 30 hrs.
Figure 4. Prestained protein ladder and serial diluted unstained protein ladder were prepared and applied in electrophoresis. After running SDS-PAGE (4-20% Novex Gel), remove the gel from the cassette then proceed to submerge the gel in a proper amount of COOMASSIEnano Protein Staining Solution, enough to cover the gel. Lightly agitate the staining box for 5 to 30 minutes at room temperature.
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Bio-Helix has been focusing on providing the best bio-reagents for life science researchers all over the world since 2007. Our products, to name a few, include DNA markers, protein markers, staining reagents, nucleic acid purification, PCR reagents and so on.
Working together with scientists in the lab around the world and continuing efforts of quality improvement, we begin to gain trusts and appraises from customers. The brand, Bio-Helix, spreading in more than forty countries, has become a trustworthy and welcome brand of reagents in laboratories of life science.
With the expansion of product lines as well as customers, we discover a greater need for a good quality supply of bio-reagents from the medical circle. Mostly, these needs are for diagnostic products. The demand of good quality bio-reagents for diagnostic devices opens a whole new possibility of innovation and motivation for Bio-Helix.
We have a vision that one day our life span can be elongated; life quality can be improved by early detection and subsequent proper treatment of all diseases. Ancient Chinese believed that a good doctor treats the disease before illness, instead of after its onset. In Bio-Helix, a new brand, LifeDirex, is created to fulfill this vision. LifeDirex will be focusing on supplying the best and most innovative reagents for diagnostic tools to promote the earliest detection of diseases as possible.
Our mission is to provide the best quality products and to develop novel technologies that assist researchers to find solutions in quest of scientific achievements. Furthermore, we decide now is the best time to enter the market of in vitro diagnostic device (IVDD). It is our goal to become the best solution provider for both research laboratory and IVDD.
Driven by desires to improve life quality and achieve scientific excellence, we operate with the highest standards of integrity. We also extend the spirit to our services and global value to our customers all around the world. A diverse workforce and network of collaboration from a great mix of company culture. Bio-Helix is not only a company of equal opportunity but also works hard to provide a friendly and growing environment.
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