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TA Cloning Kits
TA Cloning Kits
Place of Origin:
China
Brand:
Ecalbio
Model:
Supplier:
Wuhan Ecalbio Co., Ltd
Price:
Hits:
214 
Updated:
7/2/2025
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  • Product Detail
  • Company Profile
    Principle
    TA cloning kit can connect DNA fragments in an instant (a few seconds to a few minutes) and efficiently (close to 100%) under the action of Topoisomerase, so that PCR fragments are efficiently linked to carriers. TA cloning kit can connect fragments up to 10kb (that is, the insertion efficiency of 5kb fragments reaches more than 80%), which is a simple, fast, zero-background screening TOPO TA cloning vector.
     

    pCR®2.1 Preparation
    1. Prepare the solutions
     
       Fresh PCR Solution/1uL 1000bp control 0.5-8 uL
       pTOPO-T Vector 1 uL
       10’Enhancer 1 uL
       Sterile Water X uL
      10uL
     
    2. Ligation Reaction
    After adding the reagent, gently blow the mixture with a pipette or flick the bottom of the tube to mix it.
    Incubate the ligation reaction at room temperature for a minimum of 15 minutes. Longer incubation times increase the cloning efficiency.
     
    3. Centrifuge
    Collects all liquids at the bottom of the centrifuge tube based on low speed instantaneous centrifugation.
     
    ECALBIO CO.,LTD    
    Website: https: //www.ecalbio.com      
    Email: info@ecalbio.com      Tel: 0086-27-87745856
    Add : HZAU, Luoshi Road, Wuhan City 415000, China.
    bio-equip.cn
    Ecalbio Co.,Ltd specialize in developing reagents of immunodiagnostic technology and biological systems with higher accuracy andreliability for results of the highest quality.

    Ecalbio builds up professional platform to develop more than 10,000 products, including antibodies, antigens, biochemicals, ELISA kits, CLIA kits, and laboratory equipments,etc.

    Ecalbio laboratories manufacture Antibodies, Proteins, Food safety ELISA kits, Food safety Rapid Tests, Animal Disease ELISA Kits, Animal Disease Rapid Test Kits, Laboratory equipments with incubators and diagnostic system.

    Ecalbio has an enthusiastic and creative R&D team, consisting with a group of experienced technicians in lateral flow immunoassay field, and set up long relationship with first-class institutes,universities,academies, to provide excellent service for human healthy and earth.

    Ecalbio are committed to saving lives by ensuring individuals worldwide have access to timely and correct diagnosis. Our products are used in over 100 countries worldwide by:
    　Government Laboratories
    　Food industry Laboratories
    　Institutes
    　Veterinary Clinics
    　Pharmaceutical Industry.

    Ecalbio welcomes potential partners and distributors to explorebusiness relationships with all customer in the worldwide, focused on scie-tech, integrity, quality and service for the customers.

    ECALBIO CO.,LTD

    HZAU, Luoshi road, Wuhan 430060, China
    Tel:86-27-87745856
    Email:info@ecalbio.com
    Website: www.ecalbio.com
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