Description
This kit uses DNA-Only Column that can specifically bind DNA, Foregene Protease, and unique buffer system. High-quality genomic DNA can be extracted from various cultured cells and animal tissues within 30-50 minutes.
The DNA-Only silica gel membrane used in the spin column is a unique new materialfrom Foregene, which can efficiently and specifically bind DNA, remove RNA, impurity proteins, ions and other organic compounds to the maximum extent.. 5-80μg high-quality genomic DNA can be purified from 10-50mg of tissue.
Specification
|
Kit contents
|
DE-05011
|
DE-05012
|
DE-05013
|
|
50T
|
100T
|
250T
|
|
Buffer L1
|
20 mL
|
40 mL
|
100 mL
|
|
Buffer L2*
|
20 mL
|
40 mL
|
100 mL
|
|
Buffer PW*
|
25 mL
|
50 mL
|
125 mL
|
|
Buffer WB
|
25 mL
|
50 mL
|
125 mL
|
|
Buffer EB
|
10 mL
|
20 mL
|
50 mL
|
|
Foregene Protease
|
1.25 mL
|
2.5 mL
|
6.5 mL
|
|
DNA-Only Column
|
50
|
100
|
250
|
|
manual
|
1
|
1
|
1
|
*: Buffer L2 and Buffer PW contain irritating desalted salt. Please wear gloves and take relevant protective measures when operating.
Features&advantages
-No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without additional RNase during the experiment, preventing the laboratory from being contaminated by exogenous RNase.
-Fast speed: Foregene Protease has higher activity than similar proteases and digests tissue samples faster;
-Simple : the genomic DNA extraction operation can be completed in 50 minutes.
-Convenient: The centrifugation is performed at room temperature, no 4℃ low-temperature centrifugation or ethanol precipitation of DNA is required.
-Safety: no organic reagent is required.
-High quality: The purified genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, can meet the requirements of various experiments.
Kit application
It is suitable for the extraction and purification of genomic DNA from a variety of fresh or frozen cultured cells and animal tissues.
Storage and Shelf life
The kit can be stored for 12 months at room temperature (15–25 ℃) and 2–8 ℃ for longer time.
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