Scope of application
For immunoaffinity column sample preparation
Instructions
Take out the immunoaffinity chromatography column, take out the upper stopper and cut it obliquely, and insert it back on the affinity column;
----Fix the column with the glass syringe on the pump flow operation frame, and load the treated solution;
----Remove the plug under the affinity column and adjust the switch to make the liquid flow out at a speed of 1d/s;
----After the liquid is drained, wash twice with 10% methanol aqueous solution, each 10mL, with a flow rate of 2d/s;
----After the liquid is drained, load 1mL of 80% methanol water, connect the eluent with the sample bottle, and the flow rate is 1d/s;
----After elution, the liquid can be used for HPLC detection.
* Before use, the immunoaffinity chromatography column should be returned to room temperature (22~25℃).
* The last liquid should be completely drained before each sample loading.
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