REAGEN™Chloramphenicol (CAP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in fish, shrimp, eggs, honey, meat (beef, chicken and pork), milk, milk powder , condensed milk and serum.
The unique features of the kit are:
- High recovery (80-105%), rapid (10 - 40 minutes), and cost-effective extraction methods.
- High sensitivity (0.05 ng/g or ppb) and low detection limit (0.025 ng/g or ppb) for shrimp, fish and meat samples.
- High reproducibility.
- A quick ELISA assay (less than1 hour regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample and the CAP antibody are added along with secondary antibody, tagged with a peroxidase enzyme. If the CAP residue is present in the sample, it will compete for the CAP antibody, thereby preventing the antibody from binding to the CAP attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the CAP residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™Chloramphenicol (CAP) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package.
Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
|
Kit Contents
|
Amount
|
Storage
|
|
CAP Coated Plate
|
1x96-well Plate (8wells x12strips)
|
2-8°C
|
|
CAP Standards:
Negative control (white cap tube)
0.05 ng/mL (yellow cap tube)
0.15 ng/mL (orange cap tube)
0.5 ng/mL (pink cap tube)
1.5 ng/mL (purple cap tube)
4.5 ng/mL (blue cap tube)
10ng/mL(spiking, optional, red cap tube)
|
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
|
2-8°C
|
bio-equip.cn
中文
