Greater trust in your data

NGS data is increasingly being relied upon as a front line technology for the generation of data for scientific and medical research. A combination of quality metrics are used to give confidence that variants called are real and the number of false positive calls are low. The SureSeq NGS Library Preparation Kit delivers high performance in the quality metrics that really matter, giving more reliable, more trustworthy data.

Low levels of duplication

High duplication rates reduce the complexity of your NGS library and can lead to poor coverage of target genomic regions, even though average coverage rates appear high. The SureSeq NGS Library Preparation Kit gives exceptionally low levels of duplication (Figure 1). This ensures more accurate calling, more even coverage and higher levels of confidence in the data produced.

^{Figure 1: The SureSeq NGS Library Preparation Kit delivers low levels of sequence duplication. The duplication rates are shown for 8 samples sequenced using exome capture (Agilent SureSelect Human All Exon V5) with the SureSeq NGS Library Preparation Kit and a library preparation kit from an alternative supplier.}

High-quality sequence data

For complete confidence in your data, the sequence quality scores should be high and consistent across the length of each read. The SureSeq NGS Library Preparation Kit delivers exceptional sequence quality scores even 100 bases into the read (Figure 2). This reduces the requirement for post-acquisition bioinformatics, such as read size reduction, to remove poor quality sequence, giving you complete confidence in your data.

^{Figure 2: The SureSeq NGS Library Preparation Kit delivers high sequence quality. Data shown}
was generated using human genomic DNA, the OGT SureSeq Myeloid Panel and SureSeq NGS
Library Preparation Kit

High levels of on-target bases

In order to maximize the amount of useful information that can be obtained for a given sequencing lane, high levels of on-target bases are required. The benefits of superior levels of specific sequence capture include, increased sequence complexity and greater depth of coverage of the target regions. The SureSeq NGS Library Preparation Kit delivers a high percentage of on-target bases compared to other library preparation kits (Figure 3).

^{Figure 3: High percentage of on-target bases. The percentage of on-target bases are shown for exome capture (Agilent SureSelect Human All Exon V5) using the SureSeq NGS Library Preparation Kit and a library preparation kit from an alternative supplier.}

Faster process with streamlined protocol

By extensive testing of enzymes and buffer optimisation, it has been possible to streamline the standard library preparation protocol to reduce the number of hands-on steps and the overall processing time (Figure 4).

• End repair and ligation are combined into a single step
• Purification steps, where material is lost, are reduced
• Fewer steps reduce handling errors for increased reliability
• Reduced technician cost and increased sample throughput

^{Figure 4: A streamlined protocol, including enrichment by hybridisation. The complete procedure can be completed in 1.5 days with minimal handling time.}

Reliable results with full optimisation with SureSeq targeted panels and analysis software

The SureSeq NGS Library Preparation Kit works hand-in-hand with our targeted enrichment panels, which have been developed with world-leaders in their respective fields. This ensures you get the most sensitive and reproducible variant detection and industry-leading coverage uniformity.

SureSeq Interpret Software — OGT’s powerful, standalone data analysis and reporting package — is provided free with all our SureSeq targeted panels to convert FASTQ files into an intuitive interactive report. Variants are fully annotated with links to various databases (e.g. dbSNP, COSMIC, Genecards and OMIM) providing results in context.