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- Ion Exchange Chromatography Media
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Ion Exchange Chromatography Media
Cellufine MAX S, Q, CM, DEAE
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High Flow Rate, High Binding Capacity Cellufine MAX is the new, high-flow, Cellufine media. Advanced cross-linking technologies have created more robust base beads operable at high flow and pressure. Further, Cellufine MAX ion exchange (IEX) media are made using surface modification techniques that dramatically increase ligand availability, which translates to higher dynamic binding capacities. Cellufine MAX IEX media are offered in six products, including both anion and cation chemistries. |
| Cellufine MAX Base Resin | |
| Cellulose, natural polysaccharide, possesses unique crystalline molecular structure differing from non-crystalline polysaccharides such as agarose. Thus Cellufine has unique pore structure as shown in the pictograph (Fig. 1). The new Cellufine MAX series offers the largest pore size of all Cellufine chromatography media. The benefit of such pore size in Cellufine MAX IEX media provides superior strength and excellent mass transfer. This is seen in the break-through curves for thyroglobulin, a very large protein (Fig. 2). | |
 Fig 1. SEM analysis of Cellufine MAX base resin |
 Fig 2. Typical break-through curves for Cellufine DEAE weak anion exchange media with thyroglobulin |
| Partial Structure of Cellufine MAX IEX Media Ligand structure for Cellufine MAX IEX media are described in Fig. 3. S, Q, CM and DEAE are correspondingly strong cation, strong anion, weak cation and weak anion exchangers. Two sub-types, h and r, are available for Cellufine MAX S and Q. The differences between X-h and X-r type Cellufine MAX strong ion exchange media (X) are due to the design of the media. The X-h type is designed for higher binding capacity than the X-r type by optimizing the ligand content and dextran scaffold. |
 Fig 3. Ligand structure of Cellufine MAX IEX |
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| Characteristics of Cellufine MAX IEX Media | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| The basic characteristics of Cellufine MAX IEX media are shown in Table 1. All Cellufine MAX IEX media are based on 90 μm (average) highly cross-linked cellulose beads, which are surface-modified with dextran. Cellufine MAX IEX media are designed for use in bio-pharmaceuticals purification processes. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Pressure-flow Properties of Cellufine MAX IEX Media | |||
| Cellufine MAX IEX media enable high-flow operation, which is essential to efficient purification of bio-pharmaceuticals. The figures below show pressure-flow velocity curves of Cellufine MAX IEX media in a 30 cm column with a 20 cm bed height (Fig. 4). All Cellufine MAX IEX media are operable at practical flow velocities (500 cm/h) and pressures. | |||
Cellufine MAX Cation Exchange Media |
Cellufine MAX Anion Exchange Media |
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| Dynamic Binding Capacities of Cellufine MAX IEX Media |
| Efficient mass-transfer characteristics of Cellufine MAX IEX media translate to superior dynamic binding capacities (DBC). Figure 5 to 7 show DBC of model proteins at different residence times for Cellufine MAX IEX media. All Cellufine MAX IEX media are stable over a range of residence times. Fig. 8 shows that Cellufine MAX S exhibits superior dynamic binding performance across a range of protein characteristics to competitive media. These unique characteristics of Cellufine MAX IEX media make it suitable for use in up-stream as well as to down-stream steps in bio-pharmaceuticals purification. |
Cellufine MAX Cation Exchange Media |
Cellufine MAX Anion Exchange Media |
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Cellufine MAX Weak ion Exchange Media |
Cellufine MAX Cation Exchange Media |
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| Model Proteins Separation Performance for Cellufine MAX IEX Media | ||||
| Cellufine MAX IEX media are optimized for high adsorption and high resolution. Model protein separation with MAX S-h and MAX CM (Strong Cation vs. Weak Cation) is demonstrated in Fig. 9 and 10. | ||||
Cellufine MAX Cation Exchange Media |
Cellufine MAX Anion Exchange Media |
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| Chemical Stability and Cleaning-In-Place |
| Cellulose is well-known as a natural product having chemical and physical stability. Thus, since Cellufine is derived from cellulose, it also is stable to chemicals, caustic and acidic solutions. CIP of all Cellufine MAX IEX media can be carried out with 0.5 M NaOH solution. Used media should be stored in 20 % ethanol at 2 - 25 ºC after cleaning. |
| Cellufine MAX S-r |
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| Cellufine MAX S-h |
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| Cellufine MAX Q-r |
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| Cellufine MAX Q-h |
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| Cellufine MAX CM |
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| Cellufine MAX DEAE |
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View all Cellufine Chromatography Media Products
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AMSBIO’s mission is to be a profitable premier provider of quality life science research reagents and services helping customers develop innovative methods, processes, products and medicines. This is achieved by offering small and medium size manufacturers, academic groups and revenue generating biotechs a unique partnership for the global market and by providing state of the art and cost effective solutions to end users and partners.
Formed in 1987 by Sandy Allan and Alex Sim the company’s first offices were in Madrid, Spain. Over the following three years further offices were opened in Italy, Switzerland and the UK. During the formative years of existence AMS helped launch the successful sales campaigns of Stratagene and Invitrogen providing them with an important foothold from which to develop their global brand expansion.
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