Recombinant Human Activating Transcription Factor-2
=========================================================================== Antibody Arrays and Their Applications
1/Array Product Portfolio Cytokine AntiVIP (16-28) body Arrays for Human, Mouse and Rat samples Quantitative and semi-quantitative ELISA Sandwich and Label-based
Phosphorylation Antibody Arrays RTK and EGFR
Antibody Array Analysis software Protein Arrays
2/Array using protein as a capture molecule Recombinant/Purified Protein Antibody Peptide arrays Reversed phase (biological samples) Transcription factor Tissue arrays
3/Types of Cytokine Array Targets
Cytokines Inflammatory factors (interleukins, etc.) Chemokines Angiogenesis Atherosclerosis MMPs Growth Factors Adipokines Soluble receptor Signal transduction (Phosphorylation) Broad Screen (all of the above)
4/全球领先优势:
Global analysis of gene or protein expression High-throughput assays Substantially reduced costs Small sample volume
5/Antibody Arrays vs. Mass Spec
“Unbiased” = abundant Nanogram/ml vs. picogram/ml sensitivity Cheaper per sample Faster throughput Speeds up discovery process: Discovery Validation Application All in one platform
6/Antibody Arrays vs. Luminex
Affixing capture Ab reduces interactions Can screen hundreds of proteins More flexibility Equipment Mode of detection (Fluor, Chemi, Color) Less equipment maintenance
7/cDNA Arrays vs. Antibody Arrays
cDNA arrays measure only RNA levels ↑ RNA ≠↑protein levels or function Protein functions are modulated by Interactions with other proteins, DNA and/or RNA Biochemical modification of individual amino acids Antibody arrays measure protein levels Can demonstrate patterns of coupled protein expression Can detect biochemical modifications of protein Can be used to detect protein-protein, protein-DNA or protein-RNA interactions Results are more biologically meaningful
9/Features of Cytokine Antibody Array (membrane-based):
Detection Up to 274 cytokines in one experiment. Detection in picogram(pg) range. Highly specific, little cross-reactivity Handles a wide range of samples Conditioned media, plasma, serum , urine and other body fluids Cell or tissue lysates Requires low sample volumes As little as 100 μLof sample Easy to use, requires no specialized equipment Cost-effective for routine use
10/Advantages of Cytokine Array System: G-Series
Requires laser fluorescence scanner Sensitivity similar to membrane arrays Greater dynamic range Cheaper per sample than membranes High-throughput (100’s of samples/day) Small sample volume (10-50 μL/array)
BioTNT, established in 1999, is a privately held corporation located in national high-tech park of Shanghai jiangtong university longwu base. BioTNT is the leading resource for up-to-date product information, product reviews, and new technologies for worldwide life reseach scientists in the bio-technological industry. Our mission statement clearly identifies and sets the direction for our company: “ provide research products of gene tests and immunoassay tests with high quality and a very competitive price to meet the growing needs of international professionals.we aim to speed the progress of research by letting people use our technology in a manner that’s substantially more flexible than that of other tools providers.”
In the past 10 years of development, our company has formed an a wide range of products and a entire professnial technical services to help our customers succeed. We have successful developed a strong line of ELISA kit products including human, rat,mouse,pig,Canine,rabbit and Guinea Pig.Transhlod has won a extensive appreciation for our high quailty products of ELISA kit by life scientists and researchers in European countries and our company has a rich experience in export trade . Further,we also supply a series products for real-time PCR .Hot Start Taq DNA is one of our very compatitive produces. It’s would be your best choice if the requirements of PCR experiments is high. Recently ,we foucs on the research and development of PCR PreMix kit . This produce will enableus to be the leader in the field of PCR technology and to have a solid communication with the scientific community. Our empolyees desire to provide a convenient and time-saving way for customers by using our produces.
Through continuing development, BioTNT is committed to building a portfolio of quality products. Using only the highest standards, we deliver our products to the market place at highly quality and affordable prices.It is our goal to continually research and develop novel tests systems to enhance the quality of life research.