EliKine™ Human TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-α in samples. An antibody specific for TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-α present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-α is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-α bound in the initial step. The color development is stopped and the intensity of the color is measured.
The EliKine™ TNF-α Human ELISA Kit contains below components:
Human TNF-α microplate
Human TNF-α standard
Human TNF-α detect antibody
EliKine™ Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers.
The Human TNF-α ELISA Kit with the calibration range is: 7.8 pg/ml-500 pg/ml, and sensitivity is: 4 pg/mL.
More Abbkine EliKine™ series of ELISA Kits at EliKine™.
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