REAGEN™Histamine Enzymatic Assay Kit is a colorimetric enzymatic assay for the determination of histamine in fresh fish/seafood, fish meal, wine and milk.
The unique features of the kit are:
Rapid, cost-effective aqueous extraction method with high recovery.
New methanol-free extraction protocol
High sensitivity and low detection limit (6 ppm for fresh fish/seafood).
High reproducibility.
A very rapid (10 minutes) and robust enzyme-based assay which does not require chemical derivatization or expensive instrumentation.
Procedure Overview
The REAGEN™Histamine Enzymatic Assay Kit measures the chemical reduction of a dye molecule using a histamine-specific enzyme. The color change caused by reduction of the dye (measured by the absorbance at 450 nm) is related to the amount of histamine present in the sample.
Sensitivity
|
Sample Type
|
Detection Limit (ng/g or ppb)
|
|
Meat/Seafood
|
6
|
|
Fish Meal
|
10
|
|
Milk
|
40
|
|
Wine
|
40
|
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