Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit,Abbkine_specification/price/image

Product Detail
Company Profile

HCN3 conducted both potassium and sodium ions with a 3:1 preference for potassium ions. HCN3 bound cAMP, but unlike other HCNs, its activity was not modulated by intracellular cAMP.The cDNA contains a repetitive element in its 3-prime proximal region, and the deduced protein contains 703 amino acids. RT-PCR ELISA detected high HCN3 expression in adult liver and kidney and fetal liver and brain, intermediate expression in adult whole brain, lung, pancreas, spleen, testis, ovary, and all specific brain regions examined, and little to no expression in heart and skeletal muscle.Expression was high in fetal brain and adult cerebellum and intermediate in nucleus accumbens, thalamus, and pituitary gland, with little to no expression in other brain regions examined.

Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit employs a two-site sandwich ELISA to quantitate HCN3 in samples. An antibody specific for HCN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHCN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HCN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/rat-potassium-sodium-hyperpolarization-activated-cyclic-nucleotide-gated-channel-3-hcn3-elisa-kit-kte100891/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.