Lysyl oxidase is a protein. Its inhibition can cause lathyrism, but at the same time, its upregulation by tumor cells may promote metastasis of the existing tumor, causing it to become malignant and cancerous.Lysyl oxidase is an extracellular copper enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors.These aldehydes are highly reactive, and undergo spontaneous chemical reactions with other lysyl oxidase-derived aldehyde residues, or with unmodified lysine residues. This results in cross-linking collagen and elastin, which is essential for stabilization of collagen fibrils and for the integrity and elasticity of mature elastin.Complex cross-links are formed in collagen and in elastin that differ in structure.
Rat Lysyl oxidase (LOX) ELISA Kit employs a two-site sandwich ELISA to quantitate LOX in samples. An antibody specific for LOX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLOX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LOX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LOX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Lysyl oxidase (LOX) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/rat-lysyl-oxidase-lox-elisa-kit-kte100693/
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