The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth.
Rat E3 ubiquitin-protein ligase MYLIP (MYLIP) ELISA Kit employs a two-site sandwich ELISA to quantitate MYLIP in samples. An antibody specific for MYLIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYLIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYLIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYLIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat E3 ubiquitin-protein ligase MYLIP (MYLIP) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/rat-e3-ubiquitin-protein-ligase-mylip-mylip-elisa-kit-kte100549/
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