Rat 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1 (PLCZ1) ELISA Kit,Abbkine_specification/price/image

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PLCz1 Expressed specifically in testis. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis.The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion.

Rat 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1 (PLCZ1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLCZ1 in samples. An antibody specific for PLCZ1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLCZ1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLCZ1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLCZ1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1 (PLCZ1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.