In most tissues the mitochondrial ATP-synthase plays a central role by synthesizing the bulk of ATP. According to the classical theory of respiratory control, flux through this enzyme is solely determined by substrate (ADP) concentration while the enzyme has a fixed capacity.
Rabbit Neuronal mitochondrial ATPase ELISA Kit employs a two-site sandwich ELISA to quantitate Neuronal mitochondrial ATPase in samples. An antibody specific for Neuronal mitochondrial ATPase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNeuronal mitochondrial ATPase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Neuronal mitochondrial ATPase is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Neuronal mitochondrial ATPase bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rabbit Neuronal mitochondrial ATPase ELISA kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/rabbit-neuronal-mitochondrial-atpase-elisa-kit-kte90155/
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