The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini.
Pig Negative elongation factor C/D (TH1L) ELISA Kit employs a two-site sandwich ELISA to quantitate TH1L in samples. An antibody specific for TH1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTH1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TH1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TH1L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Pig Negative elongation factor C/D (TH1L) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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