Mouse Ribonuclease-like protein 13 (RNASE13) ELISA Kit,Abbkine_specification/price/image

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RNASE13 belongs to the pancreatic ribonuclease family. Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. Bovine pancreatic RNase A is one of the classic model systems of protein science.RNase A is a relatively small protein. It can be characterized as a two-layer α + β protein that is folded in half to resemble a taco, with a deep cleft for binding the RNA substrate. The first layer is composed of three alpha helices from the N-terminal half of the protein. The second layer consist of three β-hairpins arranged in two β-sheets. The hairpins 61-74 and 105-124 form a four-stranded, antiparallel β-sheet that lies on helix 3. The longest β-hairpin 79-104 mates with a short β-strand to form a three-stranded, antiparallel β-sheet that lies on helix 2.

Mouse Ribonuclease-like protein 13 (RNASE13) ELISA Kit employs a two-site sandwich ELISA to quantitate RNASE13 in samples. An antibody specific for RNASE13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRNASE13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNASE13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Ribonuclease-like protein 13 (RNASE13) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.