Arginase is the fifth and final step in the urea cycle, a series of biophysical reactions in mammals during which the body disposes of harmful ammonia. Specifically, arginase converts L-arginine into L-ornithine and urea. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. It may be found at lower levels in macrophages, lactating mammary glands, and brain. The second isozyme may be found in the absence of other urea cycle enzymes. Arginase consists of three tetramers.
Mouse Arginase-1 (ARG1) ELISA Kit employs a two-site sandwich ELISA to quantitate ARG1 in samples. An antibody specific for ARG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyARG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ARG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ARG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Arginase-1 (ARG1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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