MLEC encodes the carbohydrate-binding protein malectin which is a Type I membrane-anchored endoplasmic reticulum protein. This protein has an affinity for Glc2Man9GlcNAc2 (G2M9) N-glycans and is involved in regulating glycosylation in the endoplasmic reticulum. This protein has also been shown to interact with ribophorin I and may be involved in the directing the degradation of misfolded proteins. Alternate splicing results in multiple transcript variants.
Mouse Malectin (MLEC) ELISA Kit employs a two-site sandwich ELISA to quantitate MLEC in samples. An antibody specific for MLEC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMLEC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MLEC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MLEC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Malectin (MLEC) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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