Cartilage glycoprotein-39 is one of the major secreted proteins of ex vivo cultured human articular chondrocytes, synovial cells, and macrophages and also expressed by mature neutrophils (from myelocyte-metamyelocyte stage). High levels of YKL-40 are found in synovial fluid from patients with active rheumatoid arthritis (RA) and in the hippocampus of schizophrenic patients. YKL-40 belongs to the 18-glycosyl hydrolase (mammalian chitinase) family, and is one of the putative autoantigens in rheumatoid arthritis (RA). High levels of YKL-40 are found in synovial fluid from patients with active RA. The name 'YKL' stands for its N-terminal tripeptide sequence, and unlike the other member YKL39 which is unglycosylated, YKL40 is glycosylated.
Human Cartilage glycoprotein 39 (HC gp-39) ELISA Kit employs a two-site sandwich ELISA to quantitate HC gp39 in samples. An antibody specific for HC gp39 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHC gp39 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HC gp39 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HC gp39 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Cartilage glycoprotein 39 (HC gp-39) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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