LMO4 was expressed predominantly in the lobuloalveoli of the mammary gland during pregnancy. Consistent with a role in proliferation, forced expression of this gene inhibited differentiation of mammary epithelial cells. Overexpression of LMO4 mRNA was observed in 5 of 10 human breast cancer cell lines.Moreover, in situ hybridization analysis of 177 primary invasive breast carcinomas revealed overexpression of LMO4 in 56% of specimens. Immunohistochemistry confirmed overexpression in a high percentage (62%) of tumors. These studies implied a role for LMO4 in maintaining proliferation of mammary epithelium and suggested that deregulation of this gene may contribute to breast tumorigenesis.
Human LIM domain transcription factor LMO4 (LMO4) ELISA Kit employs a two-site sandwich ELISA to quantitate LMO4 in samples. An antibody specific for LMO4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLMO4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LMO4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LMO4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human LIM domain transcription factor LMO4 (LMO4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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