Human One cut domain family member 2 (ONECUT2) ELISA Kit,Abbkine_specification/price/image

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HNF6a encodes a member of the onecut family of transcription factors, which are characterized by a cut domain and an atypical homeodomain. The protein binds to specific DNA sequences and stimulates expression of target genes, including genes involved in melanocyte and hepatocyte differentiation. This CCDS representation uses the 5'-most in-frame start codon, which is conserved in other vertebrates, including mouse, opossum, pig and zebrafish. An alternative downstream start codon, which has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 20 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo.

Human One cut domain family member 2 (ONECUT2) ELISA Kit employs a two-site sandwich ELISA to quantitate ONECUT2 in samples. An antibody specific for ONECUT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyONECUT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ONECUT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ONECUT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human One cut domain family member 2 (ONECUT2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.