Proprotein convertase subtilisin/kexin type 7 is an enzyme belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serine endoprotease. It is structurally related to its family members, PACE and PACE4. This protein is concentrated in the trans-Golgi network, associated with the membranes, and is not secreted. It can process proalbumin and is thought to be responsible for the activation of HIV envelope glycoproteins gp160 and gp140. This gene has been implicated in the transcriptional regulation of housekeeping genes. Multiple alternatively spliced transcripts are described for this gene but their full length nature is not yet known.
Human Proprotein convertase subtilisin/kexin type 7 (PCSK7) ELISA Kit employs a two-site sandwich ELISA to quantitate PCSK7 in samples. An antibody specific for PCSK7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCSK7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Proprotein convertase subtilisin/kexin type 7 (PCSK7) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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