In yeast, the vps15 gene product is a 150-kD serine/threonine kinase that forms a complex with a phosphatidylinositol 3-kinase, vps34 (PIK3C3); in human this complex is involved in protein trafficking. By screening a cDNA library, Panaretou et al. (1997) cloned the homolog of the yeast vps15 gene, which they termed p150. The p150 cDNA encodes a predicted polypeptide of 1,358 amino acids.The amino acid sequence is 29.6% identical to that of yeast vps15 protein. Northern blot analysis revealed that the p150 gene is ubiquitously expressed as a 5.5-kb transcript. Panaretou et al. (1997) found that the p150 gene interacts with PIK3C3 in vivo.
Human Phosphoinositide 3-kinase regulatory subunit 4 (PIK3R4) ELISA Kit employs a two-site sandwich ELISA to quantitate PIK3R4 in samples. An antibody specific for PIK3R4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIK3R4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3R4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3R4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Phosphoinositide 3-kinase regulatory subunit 4 (PIK3R4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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