Tamagnone et al. (1999) identified the cDNA sequence of a novel member of the plexin gene family and named the gene plexin D1. Plxnd1 was expressed by embryonic vascular endothelial cells. Expression was robust in endocardium, intersomitic vessels, pulmonary vasculature, aorta, and pharyngeal arch arteries. In adult mice, Plxnd1 was expressed in heart, brain, lung, kidney, and testis, consistent with broad expression in endothelial cells. Human vascular endothelial cells also expressed PLXND1. Gitler et al. (2004) demonstrated that mouse Plxnd1 heterodimerized with Npn1 to form a functional Sema3C receptor. Binding of Sema3A and Sema3C to COS cells expressing Npn1 was enhanced by cotransfection of Plxnd1. Plxnd1 also enhanced binding of Sema3C to Npn2.
Human Plexin-D1 (PLXND1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLXND1 in samples. An antibody specific for PLXND1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLXND1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXND1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXND1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Plexin-D1 (PLXND1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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