Human DNA-directed RNA polymerase II subunit RPB11-b1 (POLR2J2) ELISA Kit,Abbkine_specification/price/image

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POLR2J2 is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo.

Human DNA-directed RNA polymerase II subunit RPB11-b1 (POLR2J2) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR2J2 in samples. An antibody specific for POLR2J2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLR2J2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR2J2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR2J2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human DNA-directed RNA polymerase II subunit RPB11-b1 (POLR2J2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.