Apolipoprotein L (APOL) proteins belong to the high density lipoprotein family, which plays a central role in cholesterol transport. The cholesterol content of membranes is important in cellular processes such as modulating gene transcription and signal transduction both in the adult brain and during neurodevelopment. The 6 APOL genes are clustered on chromosome 22q12.3.The cDNA encodes a 383-amino acid polypeptide that includes a 12-amino acid secretory signal peptide. Northern blot analysis detected a 1.3-kb APOL transcript in the pancreas but not in any other human tissues. Affinity immunosorption showed that APOL is not free in the plasma, but is associated with lipoproteins containing APOA1. APOL was found in high density lipoprotein fractions.
Human Apolipoprotein L1 (APOL1) ELISA Kit employs a two-site sandwich ELISA to quantitate APOL1 in samples. An antibody specific for APOL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAPOL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Apolipoprotein L1 (APOL1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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