Human Thrombin activatable fibrinolysis inhibitor (TAFI) ELISA Kit,Abbkine_specification/price/image

Product Detail
Company Profile

TAFI, Thrombin Activatable Fibrinolytic Inhibitor, (also known as carboxypeptidase U and plasma carboxypeptidase B) is a 60,000 D molecular ratio glycoprotein (proenzyme form) present in rabbit plasma1 that modulates fibrinolysis in vivo. This proenzyme is converted to a 35,000 D molecular ratio active form, TAFIa, following proteolytic cleavage by the thrombin/ thrombomodulin complex. TAFIa possesses carboxypeptidase activity with a preference for cleaving lysine and arginine residues from the c-terminus of proteins. Modulation of fibrinolysis occurs when TAFIa cleaves C-terminal arginine and lysine residues of partially degraded fibrin.2,4,5 The removal of the c-terminus arginine and lysine residues from fibrin inhibits the continued degradation of fibrin by tPA activated plasmin.3

Human Thrombin activatable fibrinolysis inhibitor (TAFI) ELISA Kit employs a two-site sandwich ELISA to quantitate CPB2 in samples. An antibody specific for CPB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCPB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Thrombin activatable fibrinolysis inhibitor (TAFI) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-thrombin-activatable-fibrinolysis-inhibitor-tafi-elisa-kit-kte62275/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.