Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator.This enzyme is a tetramer composed of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency.The predicted protein is 458 amino acids long. Carboxypeptidase N is a 280-kD tetrameric complex consisting of 2 identical 83-kD regulatory subunits and 2 identical 50-kD catalytic subunits.
Human Carboxypeptidase N catalytic chain (CPN1) ELISA Kit employs a two-site sandwich ELISA to quantitate CPN1 in samples. An antibody specific for CPN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCPN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Carboxypeptidase N catalytic chain (CPN1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-carboxypeptidase-n-catalytic-chain-cpn1-elisa-kit-kte62274/
bio-equip.cn
中文
