The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission.The deduced 152-amino acid protein contains a central leucine zipper, a coiled-coil region, and a C-terminal transmembrane domain. It also contains a region showing high interspecies homology and a putative tetratricopeptide repeat. Immunocytochemical analysis of HeLa cells detected endogenous FIS1 expression in mitochondria. Protease treatment indicated that FIS1 is expressed at the outer mitochondrial membrane. Transfection of a C-terminal truncated FIS1 resulted in cytoplasmic expression, indicating that the C terminus is required for mitochondrial localization.
Human Mitochondrial fission 1 protein (FIS1) ELISA Kit employs a two-site sandwich ELISA to quantitate FIS1 in samples. An antibody specific for FIS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFIS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FIS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FIS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Mitochondrial fission 1 protein (FIS1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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