Human Fibronectin type III domain-containing protein 5 (FNDC5) ELISA Kit,Abbkine_specification/price/image

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FNDC5 contains an N-terminal signal peptide, a fibronectin type III domain, and a transmembrane domain. The 2 proteins share 60% identity in the FNIII domain and 86% in the transmembrane domain. Northern blot analysis of multiple mouse tissues detected highest expression of Fndc5 in adult brain and heart, and low expression in lung, skeletal muscle, and testis. Northern blot analysis of mouse embryonic mRNA first detected Fndc5 expression at embryonic day 11, with strongest expression at E15 and decreasing expression at E17. In situ hybridization demonstrated expression of Fndc5 in the fore- and midbrain, as well as in the brachial arches and the heart. Overexpression of a GFP-Fndc5 fusion protein in COS cells showed localization predominantly in the endoplasmic reticulum, with small amounts in the plasma membrane.

Human Fibronectin type III domain-containing protein 5 (FNDC5) ELISA Kit employs a two-site sandwich ELISA to quantitate FNDC5 in samples. An antibody specific for FNDC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFNDC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FNDC5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FNDC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Fibronectin type III domain-containing protein 5 (FNDC5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.