Human Inositol 1,4,5,-trisphosphate (IP3) ELISA Kit,Abbkine_specification/price/image

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Inositol trisphosphate is a secondary messenger molecule used in signal transduction in biological cells. It is made by hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2), a phospholipid that is located in the plasma membrane, by phospholipase C. IP3 binds to and activates the InsP3 receptor on the membrane of the endoplasmic reticulum (ER), and sarcoplasmic reticulum (SR) opens a calcium channel, resulting in the release of Ca2 into the cytoplasm, and sarcoplasm respectively. This increase in Ca2 activates the ryanodine receptor-operated channel on the SR, leading to a further increase in the Ca2 .Its main functions are to mobilize Ca2+ from storage organelles and to regulate cell proliferation and other cellular reactions.In smooth muscle cells, for example, the increase in concentration of cytoplasmic calcium results in the contraction of the muscle cell.

Human Inositol 1,4,5,-trisphosphate (IP3) ELISA Kit employs a two-site sandwich ELISA to quantitate IP3 in samples. An antibody specific for IP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Inositol 1,4,5,-trisphosphate (IP3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-inositol-145-trisphosphate-ip3-elisa-kit-kte62242/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.