Dihydropyrimidinase catalyzes the conversion of 5,6-dihydrouracil to 3-ureidopropionate in pyrimidine metabolism. Dihydropyrimidinase is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria.Various neurologic abnormalities have been described in this group of patients. The further catabolism of the dihydropyrimidines is effected by dihydropyrimidinase. Duran et al. reported a male Turkish baby, the fifth child of a consanguineous couple, who was thought to have dihydropyrimidinase deficiency. The infant was well until the age of 8 weeks when he developed feeding problems following the introduction of fruits to his formula milk.
Human Dihydropyrimidinase (DPYS) ELISA Kit employs a two-site sandwich ELISA to quantitate DPYS in samples. An antibody specific for DPYS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPYS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPYS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPYS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Dihydropyrimidinase (DPYS) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-dihydropyrimidinase-dpys-elisa-kit-kte61981/
bio-equip.cn
中文
