Ephrin-A1 is a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis.
Human Ephrin-A1 (EFNA1) ELISA Kit employs a two-site sandwich ELISA to quantitate EFNA1 in samples. An antibody specific for EFNA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEFNA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFNA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFNA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Ephrin-A1 (EFNA1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-ephrin-a1-efna1-elisa-kit-kte61950/
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