MUC21 encodes a large membrane-bound glycoprotein which is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. The encoded protein contains an N-terminal signal sequence, an extracellular mucin domain, a stem domain, a transmembrane domain, and a C-terminal cytoplasmic tail domain. The mucin domain contains O-glycosylation sites and is polymorphic with isoforms containing a variable number of nonidentical proline-, threonine-, and serine-rich tandem repeats of 15 amino acids each. The aberrent expression of MUC21 is associated with lung adenocarcinoma.
Human Mucin-21 (MUC21) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC21 in samples. An antibody specific for MUC21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC21 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC21 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Mucin-21 (MUC21) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-mucin-21-muc21-elisa-kit-kte61456/
bio-equip.cn
中文
