Product introduction：
The Bio-Gener iCellBox, in combination with a -80℃freezer or dry ice locker, will provide the freezing rate of -1 degree perminute that is ideal for cryo-preservation of most cultured cell lines. TheCellHome design user a combination of insulation foam, radial symmetry, and aheat transfer core to regulate heat loss rather than using a large thermal mass(alcohol based freezer).
Key Features:
1. The 12 chambers and sample tubes should be dry to avoid tube sticking upon freezing．
2. Make sure the core (metal ring) is at room temperature and seated in the bottom of the central cavity.
3. Place sample vials containing maxinum 2 ml of cell suspension in each well .The sample vials should not extend about the iCellbox body.
4. Check that the tubes slide in and out freely.
5. Fully seat the lid on the iCellbox.
6. Put the iCellbox upright into a -80℃ freezer or dry ice locker. Ensure that there is at least 1 inch of free space clearance around the iCellbox.
7. Freeze for 4 hours before transferring samples to archive storage.
Parameters:
The iCellbox will freeze 12 tubes each containing maxinum 2 ml of cell suspension at -1℃ per minute when placed in a -80℃ environment (mechanicl freezer or dry ice locker). The five consecutive run freezing profile curves at right were performed wish 12 sample loads each.
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