MTNR1B encodes the MT2 protein, one of two high-affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This gene product is an integral membrane protein that is a G-protein coupled, 7-transmembrane receptor. It is found primarily in the retina and brain; however, this detection requires RT-PCR. It is thought to participate in light-dependent functions in the retina and may be involved in the neurobiological effects of melatonin. Besides the brain and retina this receptor is expressed on the bone forming cells where it regulates their function in depositing bone.
Rat Melatonin receptor type 1B (MTNR1B) ELISA Kit employs a two-site sandwich ELISA to quantitate MTNR1B in samples. An antibody specific for MTNR1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTNR1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTNR1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTNR1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Melatonin receptor type 1B (MTNR1B) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/rat-melatonin-receptor-type-1b-mtnr1b-elisa-kit-kte100564/
bio-equip.cn
中文
