Nuclear receptor coactivator 6 encoded by NCOA6 is a transcriptional coactivator that can interact with nuclear hormone receptors to enhance their transcriptional activator functions. Nuclear receptor coactivator 6 has been shown to be involved in the hormone-dependent coactivation of several receptors, including prostanoid, retinoid, vitamin D3, thyroid hormone, and steroid receptors. Alternatively spliced transcript variants encoding different isoforms have been described for NCOA6.
Rat Nuclear receptor coactivator 6 (NCOA6) ELISA Kit employs a two-site sandwich ELISA to quantitate NCOA6 in samples. An antibody specific for NCOA6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCOA6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCOA6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCOA6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Nuclear receptor coactivator 6 (NCOA6) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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