Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. Human β-glucuronidase is a type of glucuronidase (a member of glycosidase Family 2) that catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides (also referred to as glycosaminoglycans) such as heparan sulfate. Human β-glucuronidase is located in the lysosome. In the gut, brush border β-glucuronidase converts conjugated bilirubin to the unconjugated form for reabsorption. Beta-glucuronidase is also present in breast milk, which contributes to neonatal jaundice. The protein is encoded by the GUSB gene.
Human Putative beta-glucuronidase-like protein SMA3 (GUSBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate GUSBP1 in samples. An antibody specific for GUSBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGUSBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GUSBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GUSBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Putative beta-glucuronidase-like protein SMA3 (GUSBP1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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