TPI1 encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants.Spliceosomal introns are present in the nuclear protein-coding genes of most eukaryotic organisms, but they have not been detected in several eukaryotic protist phyla or in eubacteria, archaebacteria, and organelles. Two major theories had emerged in the continuing debate on the origin of these introns.
Human Triosephosphate isomerase (TPI1) ELISA Kit employs a two-site sandwich ELISA to quantitate TPI1 in samples. An antibody specific for TPI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Triosephosphate isomerase (TPI1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-triosephosphate-isomerase-tpi1-elisa-kit-kte62315/
bio-equip.cn
中文
